Setup of 3‑D Life and ToLuminate Hydrogels

Use this form to calculate the volumes of gel components required for the setup of various hydrogel compositions.

For detailed information please refer to the General Protocols for each type of gel.

  1. Section 1: Please enter the type of crosslinking chemistry and the type of crosslinker. Note that there are two different stock concentrations of crosslinkers, which will autmatically be entered according to your selection.
  2. Section 2: The standard stock concentrations of your reagents are already entered. Update, if you use stocks with different concentrations.
  3. Section 3: Enter the final crosslinking concentration and, optionally, enter the final concentration of AgaFloat, added peptides and/or Thioglycerol.
  4. Section 4: Enter the volume of your biological sample (e. g. cell suspension, tissue fragments) and the total hydrogel volume.
  5. Click "Calculate Volumes" to receive your pipetting protocol. Click again after each change of hydrogel composition.
  6. Red bordered fields are mandatory to allow the calculation of a gel composition.

Section 1: Define the type of crosslinking:

Crosslinking chemistry:



Type of crosslinker:

Section 2: Enter stock concentrations:

Thiol-reactive polymer:
  mmol/L
Crosslinker:
  mmol/L
RGD Peptide:
  mmol/L
GFOGER-3 Peptide:
  mg/mL
Thioglycerol:
  mmol/L

Section 3: Enter final concentrations

Crosslinking concentration:

  mmol/L

AgaFloat:
  %
RGD Peptide:
  mmol/L
GFOGER-3 Peptide:
  mg/mL
Thioglycerol:
  mmol/L

Section 4:

Add a biological sample:
  µl
Enter final volume:
  µl

 

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